Present affiliation: NASI Senior Scientist
Department of Biotechnology
All India Institute of Medical Sciences
New Delhi-110029
and Honorary Professor
THSTI, Faridabad, Haryana
Email ID: jstyagi@aiims.edu
Phone(O): +91-11-26593549
Education:
B.Sc. (1973), M.Sc. (1975), Ph.D. (1979), University of Delhi; Post-doctoral Fellowship and Associateship, National Cancer Institute, NIH, USA (1979-1983).
Teaching and Research experience:
1) Teaching: Ph.D., Masters in Biotechnology students, trainees
2) Research: 37 years research experience in Molecular mycobacteriology, TB pathogen biology and Technology Development.
3) Focus of Research:
Discovery and characterization of DevR-DevS system in M. tuberculosis essential for dormancy; Establishing proof-of-concept for DevR as a dormancy drug target; Discovery and elucidating
mechanisms underlying vitamin C’s dormancy-inducing activity in M. tuberculosis; Demonstrating vitamin C’s potential in adjunctive TB therapy, developing screening systems for discovery of
anti-dormancy compounds; Developing and validating TB diagnostic technologies and kits.
Fellowships/Honours/Awards:
Fellow, Indian National Science Academy (New Delhi)
Fellow, Indian Academy of Sciences (Bangalore)
Fellow, National Academy of Sciences of India (Allahabad)
Elected member, Guha Research Conference
Janaki Ammal National Women Bioscientist Award
AIIMS Excellence Research Award 2018 First Prize in Basic Research category
Prof. (Mrs.) Archana Sharma Memorial Lecture Award (NASI)
Professor Jyotirmoy Das Memorial Lecture Award (IICB)
Stree Shakti Science Samman
J.C. Bose National Fellowship
DBT Tata Innovation Fellowship
Dr. Kona Sampath Kumar Memorial Prize, University of Delhi
P.S. Sarma Memorial Award (SBCI)
DBT National Women Bioscientist Award (Young category)
New Millennium Science Medal, Indian Science Congress
Nandi M, Sikri K, Chaudhary N, Mande SC, Sharma RD, Tyagi JS (2019) Multiple transcription factors co-regulate the Mycobacterium tuberculosis adaptation response to vitamin C. BMC Genomics 20:887. https://doi.org/10.1186/s12864-019-6190-3
Gautam US, Mehra S, Kumari P, Alvarez X, Niu T, Tyagi JS, Kaushal D. (2019) Mycobacterium tuberculosis sensor kinase DosS modulates the autophagosome in a DosR-independent manner. Communications biology. 2:349. Epub 2019/09/26.
Anthwal D, Lavania S, Gupta RK, Verma A, Myneedu VP, Sharma PP, Verma H, Malhotra V, Gupta A, Gupta NK, Sarin R, Haldar S, Tyagi JS (2019) Development and evaluation of novel bio-safe filter paper-based kits for sputum microscopy and transport to directly detect Mycobacterium tuberculosis and associated drug resistance. PLoS ONE 14(8): e0220967. https://doi.org/10.1371/ journal.pone.0220967
Mishra SK, Shankar U, Jain N, Sikri K, Tyagi JS, Sharma TK, Mergny J, Kumar A (2019) Characterization of G-quadruplex motifs in espB, espK and cyp15 genes of Mycobacterium tuberculosis as a potential drug targets. Molecular Therapy Nucleic acids. 16: 698–706.
Dhiman A, Kumar C, Mishra SK, Sikri , Datta I, Sharma P, Singh TP, Haldar S, Sharma N, Bansal A, Ahmad Y, Kumar A, Sharma TK, Tyagi JS (2019) Theranostic Application of a Novel G-Quadruplex-Forming DNA Aptamer Targeting Malate Synthase of Mycobacterium tuberculosis. Molecular Therapy Nucleic acids 18:661-672. doi: 10.1016/j.omtn.2019.09.026
De Majumdar S, Sikri K, Ghosh P, Jaisinghani N, Nandi M, Gandotra S, Mande S, Tyagi JS (2019) Genome analysis identifies a spontaneous nonsense mutation in ppsD leading to attenuation of virulence in laboratory-manipulated Mycobacterium tuberculosis. BMC Genomics http://doi.org/10.1186/s12864-019-5482-y
Sharma S, Kumari P, Vashist, Kumar C, Nandi M and Tyagi JS (2018) Cognate sensor kinase-independent activation of Mycobacterium tuberculosis response regulator DevR (DosR) by acetyl phosphate: Implications in anti-mycobacterial drug design. Molecular Microbiology, https://doi.org/10.1111/mmi.14196
Das R, Dhiman A, Mishra SK, Haldar S, Sharma N, Bansal A, Ahmad Y, Kumar A, Tyagi JS, Sharma TK (2018) Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis. International Journal of Nanomedicine, doi: 10.2147/IJN.S189127
Lavania S, Das R, Dhiman A, Myneedu V, Verma A, Singh N, Sharma TK, Tyagi JS (2018) Aptamer-based TB antigen tests for the rapid diagnosis of pulmonary tuberculosis: Potential utility in screening for tuberculosis. ACS Infectious Diseases doi: 10.1021/acsinfecdis.8b00201
i. Link to media coverage:
ii.
https://www.thehindu.com/sci-tech/aiims-led...for...pleural-tb/article25463672.ece
Kumari P, Lavania S, Tyagi S, Dhiman A, Rath D, Anthwal D, Gupta RK, Sharma N, Gadpayle AK, Taneja RS, Sharma L, Ahmad Y, Sharma TK, Haldar S, Tyagi JS (2018) A novel aptamer-based test for the rapid and accurate diagnosis of pleural tuberculosis. Analytical Biochemistry 564-565:80-87.
https://doi.org/10.1016/j.ab.2018.10.019
Vashist A, Malhotra V, Sharma G, Tyagi JS, Clark-Curtiss J-E. (2018) Interplay of PhoP and DevR response regulators defines expression of the dormancy regulon in virulent Mycobacterium tuberculosis. J. Biol. Chem. 293(42):16413-16425 doi: 10.1074/jbc.RA118.004331jbc.RA118.004331
Kalra P, Mishra SK, Kaur S, Kumar A, Prasad HK, Sharma TK, Tyagi JS (2018) G-quadruplex-forming DNA aptamers inhibit DNA-binding function of HupB and Mycobacterium tuberculosis entry into host cells. Molecular Therapy - Nucleic Acids. https://www.sciencedirect.com/science/article/pii/S2162253118302233
Dhiman A, Haldar S, Mishra SK, Sharma N, Bansal A, Ahmad Y, Kumar A, Sharma TK, Tyagi JS (2018) Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitis. Tuberculosis. 112:27-36.
Sikri K, Duggal P, Kumar C, Batra SD, Vashist A, Bhaskar A, Tripathi K, Sethi TP, Singh A, Tyagi JS (2018) Multifaceted remodeling by vitamin C boosts sensitivity of Mycobacterium tuberculosis subpopulations to combination treatment by anti-tubercular drugs. Redox Biology.15:452-466.
Lavania S, Anthwal D, Bhalla M, Singh N, Haldar S, Tyagi JS (2017) Direct detection of Mycobacterium tuberculosis rifampin resistance in bio-safe stained sputum smears. PLOS ONE, 12(12):e0189149. doi: 10.1371/journal.pone.0189149
Tyagi S, Sharma N, Tyagi JS, Haldar S. (2017) Challenges in pleural tuberculosis diagnosis: existing reference standards and nucleic acid tests. Future Microbiol. 12:1201-1218. doi: 10.2217/fmb-2017-0028
Kumari P, Sikri K, Kaur K, Gupta UD, Tyagi JS (2017) Sustained expression of DevR/DosR during long-term hypoxic culture of Mycobacterium tuberculosis. Tuberculosis (Edinb). 106:33-37.
Batra SD, Nandi M, Sikri K, Tyagi JS. (2017) Genome-wide expression profiling establishes novel modulatory roles of vitamin C in THP-1 human monocytic cell line. BMC Genomics 18(1):252. doi: 10.1186/s12864-017-3635-4.
Anthwal D, Gupta RK, Bhalla M, Bhatnagar S, Tyagi JS, Haldar S. (2017) Direct Detection of Rifampin and Isoniazid Resistance in Sputum Samples from Tuberculosis Patients by High-Resolution Melt Curve Analysis. J Clin Microbiol. 55(6):1755-1766. doi: 10.1128/JCM.02104-16
Sharma S, Tyagi JS (2016) Mycobacterium tuberculosis DevR/DosR Dormancy Regulator Activation Mechanism: Dispensability of Phosphorylation, Cooperativity and Essentiality of α10 Helix. PLoS ONE 11(8): e0160723. doi:10.1371/journal.pone.0160723
Kaur K*, Priyanka Kumari*, Sharma S, Sehgal S, Tyagi JS (2016) DevS/DosS sensor is bifunctional and its phosphatase activity precludes aerobic DevR/DosRregulon expression in Mycobacterium tuberculosis. The FEBS Journal 283, 2949–2962.
Nathan C, Bahr NC, Marais S, Caws M, van Crevel R, Wilkinson R, Tyagi JS, Thwaites GE, Boulware DR (2016) GeneXpert MTB/Rif to Diagnose Tuberculous Meningitis: Perhaps the First Test but not the Last. Clinical Infectious Diseases doi:10.1093/cid/ciw083
Vashist A, D. Prithvi Raj, Gupta UD, BhatR, Tyagi JS (2016) The α10 helix of DevR, the Mycobacterium tuberculosis dormancy response regulator, regulates its DNA binding and activity. The FEBS Journal 283, 1286–1299
#Tyagi JS (2016) The TB battle: How well are we fighting the bug? Nature India, 42-44
Thakral P, Singla S, Vashist A, Yadav MP, Gupta SK, Tyagi JS, Sharma A, Bal CS, Snehlata EY, Malhotra A (2016) Preliminary Experience with Yttrium-90-labelled Rituximab (Chimeric Anti CD-20 Antibody) in Patients with Relapsed and Refractory B Cell Non-Hodgkins Lymphoma. Curr Radiopharm. 9(2):160-8.
Sikri K, Batra SD, Nandi M, Kumari P, Taneja NK, Tyagi JS (2015) The pleiotropic transcriptional response of Mycobacterium tuberculosis to vitamin C is robust and overlaps with the bacterial response to multiple intracellular stresses. Microbiology 161, 739–753
Patents Granted
P1. A process for identifying and producing DevR protein of Mycobacterium tuberculosis. Indian patent granted -184437
P2. A rapid efficient single-tube extraction procedure for the isolation of PCR-amplifiable M. tuberculosis DNA from clinical specimens. Indian patent granted -191511
P3. A method for preparation and preservation of clinical samples for microbial infections.Granted -228304 (old name 202383), Indian patent
P4. Involvement of a two-component system devR-devS (Rv3133c-Rv3132c) in the virulence of M. tuberculosis: Potential as a new antitubercular drug target. Indian patent granted -19375
P5. A screening method for developing drugs against pathogenic microbes having two-component system. Filed- Indian, US, PCT [published]
P6. DevR-DevS and DevR-2027c signal transduction systems as targets for anti-tubercular therapy. Patent granted – EU (DE, EP, FR, GB) EL1554586; US 7566550, CN ZL200380104539.1, WO Appl. No,.PCT/IN03/04555; IN Appl. No. 128/DEL2003 [FN: 7856].
P7. A process for identifying a novel target for use for the development of therapeutic modalities and drugs effective against tuberculosis. Granted -UK GB 2398302; Russian 2280693; EP 1472339 (GERMANY 602 25 722.0-08, FRANCE, SWEDEN), US 7332340 B2, Japan 4229238
P8. A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof. [Indian patent granted, National phase filed in China, Europe, US and ARIPO countries] Granted- EP 1897957, EP 16949014; designated in Germany, France,Great Britain, Italy.
P9. A simple and fast process for evaluating Mycobacterium tuberculosis promoters and the effect of candidate antimycobacterial compounds on promoter activity and bacterial viablility under hypoxic and aerobic conditions using M. smegmatis as a surrogate host.” Indian patent granted - 211217.
Patents Filed
P1. Novel Aptamer based Detection System for Tuberculous Meningitis. Indian Provisional Patent application no. 201611001550, filed on 15.01.2016
P2. Isolated single stranded polynucleotides and uses thereof in diagnosis and treatment of Tuberculous Meningitis. Indian Provisional Patent Application no.201611021901, filed on 27.06.2016.
P3. Aptamer based diagnostic and therapeutic agent for and against HupB of Mycobacterium tuberculosis. Indian Provisional Patent Application no.201711001246 , filed on 12.01.2017.
